Key Ideas
1Central dogma. DNA → (transcription) → mRNA → (translation) → Protein.
2Transcription. In the nucleus by RNA polymerase; template read 3'→5', mRNA made 5'→3'; mRNA uses uracil not thymine.
3Translation. At 80S ribosomes; tRNA carries amino acids; codon = 3 bases = 1 amino acid.
4Mutations. Point = 1 base change. Frameshift = insertion/deletion shifting every codon after it.
5Sickle cell disease. GAG→GTG point mutation swaps Glu→Val in beta-globin; carriers gain malaria resistance.