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Biology Worksheet

🧬 DNA Structure & Replication

Chapter: Genetics & DNA · Level ★★☆ · Time: 25 min
SCORE___ / 20
NameClassDate
After this worksheet you can
State base-pairing rulesName replication enzymesDistinguish leading/lagging strandState the central dogma
📚 Quick Recap

🎯 What you'll practice: DNA structure, base pairing, semi-conservative replication, and the enzymes involved.

🧠 Section A · Concept Check ● BEGINNER 4 × 1 = 4

1Adenine pairs with:
2Which enzyme unwinds the double helix?
3Okazaki fragments form on the:
4The central dogma order is:

🧮 Section B · Problem Solving ● INTERMEDIATE 2 + 3×3 = 11

5G≡C is held by hydrogen bonds.
6 joins Okazaki fragments together.
7Why is DNA replication called "semi-conservative"?
8What does primase do?
9A DNA strand reads 5'-ATGCC-3'. Write its complementary strand.

🚀 Section C · Challenge ● CHALLENGE 5

10DNA polymerase can only add nucleotides in the 5'→3' direction. Explain why this single fact forces one strand to be synthesised continuously and the other in fragments.
💭 Reflection — the most useful thing I learned:
A ___/4   B ___/11   C ___/5   Total ___/20 Teacher's Signature Parent's Signature
✂ answer key — fold or cut before handing out

1-C   2-B   3-B   4-B  |  5 = 3   6 = ligase   7 = because each of the two resulting DNA molecules contains one original (template) strand and one newly synthesised strand, rather than being either fully new or fully old   8 = it lays down a short RNA primer that gives DNA polymerase a starting point to begin adding nucleotides   9 = 3'-TACGG-5'  |  10 = Because the two template strands run antiparallel to each other, only one of them is oriented so DNA polymerase can add nucleotides continuously toward the replication fork in the 5'→3' direction (the leading strand); the other template strand runs the opposite way relative to the fork, so DNA polymerase must repeatedly start, extend a short stretch, and stop — creating Okazaki fragments — because it can never build directly toward the fork on that strand in the 5'→3' direction.

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